Practicum in biohysics 1100-1BB29
The course covers the examination of the diffraction, interference and dispersion of electromagnetic radiation as well as the investigation of the processes of absorption and emission of light. Students learn how to use UV/Vis spectrophotometer and a spectrofluorimeter.
During the classes, students perform the following exercises:
1. Investigation of diffraction and interference of laser light
2. Examination of optical spectra with a spectrometer
3. Measurements of absorption spectra of food dye solutions
4. Examination of the Lambert-Beer law
5. Measurements of absorption and fluorescence spectra of fluorescent compounds
Estimated time of work:
participation in classes – 15 h
preparation for classes – 10 h
preparation of reports – 20 h
Total – 45 h
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
A successful student:
Knowledge:
1. knows the physical basis of the absorption and fluorescence phenomena
2. knows how the spectrometer, UV/Vis spectrophotometer and spectrofluorimeter work
3. knows the basic concepts and laws related to UV/Vis spectroscopy
Skills:
1. can use basic equipment useful in biophysical studies
2. can explain the relationship between the absorbed energy and the structure of the molecule as well as its color
3. can determine the sizes of very small objects based on diffraction and interference of the laser light
4. is able to analyse and present the experimental results and can describe the observations and the conclusions
Attitudes:
1. is aware of the responsibility for own experimental results
2. takes care of the safety work with laser light sources, chemical and biological substances and equipment used in biophysical research
Assessment criteria
Students perform 5 laboratory exercises, each documented with an individual report. For completing the course it is necessary to obtain positive grades for all reports. The final score is calculated as the arithmetic mean of partial notes.
Class attendance is obligatory. One excusable absence is allowed.
Bibliography
1. D. Halliday, R. Resnick, J. Walker, „Podstawy fizyki”, t. 4-5, PWN
2. P. Atkins, Julio de Paula „Chemia fizyczna” PWN 2016
3. Z. Jóźwiak, G. Bartosz, „Biofizyka. Wybrane zagadnienia wraz z ćwiczeniami”, PWN 2007
4. J. Sadlej, „Spektroskopia molekularna”, WNT 2002.
5. Z. Kęcki, „Podstawy spektroskopii molekularnej”, PWN 1998
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: